510-533_HPLCapps.qxp
11/19/2008
3:16 PM
Page 526
HPLC APPLICATIONS | PHARMACEUTICAL
Antibiotics Antibiotic Sulfamethoxazole on Allure® Basix
Antibiotic Sulfamethoxazole, Trimethoprim, and Naphthoate on Allure® C18
1
Peak List: 1. trimethoprim 2. sulfamethoxazole 3. naphthoate
1
Peak List: 1. sulfamethoxazole Sample: Inj.: Conc.: Solvent: Column: Cat.#: Dimensions: Particle size: Pore size: Conditions: Mobile phase: Flow rate: Temp.: Det.:
Sample: Inj.: Conc.: Solvent:
2
10µL 100µg/mL water:methanol (1:1, v/v) Allure® Basix 9161565 150mm x 4.6mm 5µm 60Å
10µL 100µg/mL water:methanol (1:1, v/v)
Column: Cat.#: Dimensions: Particle size: Pore size: Conditions: Mobile phase:
water:methanol (70:30, v/v) 1.0mL/min. ambient UV @ 254nm
Flow rate: Temp.: Det.:
Allure® C18 9164565 150mm x 4.6mm 5µm 60Å 10mM ammonium formate, pH to 3.0 with formic acid: methanol (50:50, v/v) 1.0mL/min. ambient UV @ 220nm
3
0
2.5
5
7.5
10
12.5
15
17.5 min. 0
2
4
6
LC_0066
Tetracycline Mix on Allure® Biphenyl Ret. Time (min.) 4.59 5.19 6.32 11.16 15.81 19.66
Peak List 1. oxytetracycline 2. degradation peak 3. tetracycline 4. degradation peak 5. chlortetracycline 6. doxycycline
Sample: Inj.: 30µL Conc.: 100µg/mL each component Sample diluent: methanol Column: Cat.#: Dimensions: Particle size: Pore size: Conditions: Mobile phase: Flow: Temp.: Det.:
Allure Biphenyl 9166565 150mm x 4.6mm 5µm 60Å ®
20mM ammonium phosphate (pH 2.5):acetonitrile, 80:20 1mL/min. ambient UV @ 254nm
LC_PH0347
526 www.restek.com
8
10
12
14 min.
LC_0067
Oxytetracycline and Tetracycline on Allure® Biphenyl
Peak List 1. oxytetracycline 2. degradation peak 3. tetracycline
Ret. Time (min.) 4.92 5.66 7.10
Sample: Inj.: 20µL Conc.: 100µg/mL each component Sample diluent: methanol Column: Cat.#: Dimensions: Particle size: Pore size: Conditions: Mobile phase: Flow: Temp.: Det.:
Allure® Biphenyl 9166565 150mm x 4.6mm 5µm 60Å 20mM ammonium phosphate (pH 2.5):acetonitrile, 80:20 1mL/min. ambient UV @ 254nm
LC_PH0340
800-356-1688 or 814-353-1300
